一、 简历
1983年毕生于南京大学生物系生物化学专业，同年任教于沈阳药科大学生物化学教研室。在南京大学生命科学院生物化学系―医药生物技术国家重点实验室毕业并获生化与分子生物学理学博士学位。在沈阳药科大学药学博士后工作站从事药学科研工作两年。
二、职务及学术兼职
沈阳药科大学医疗器械学院院长
沈阳药科大学学位委员会委员；生物学分科主席
辽宁省生化与分子生物学学会理事长
辽宁省医学信息与健康工程学会副理事长
辽宁省生物物理学会副理事长
辽宁省细胞生物学会常务理事
辽宁省经济文化发展促进会常务理事
辽宁省生物技术协会理事；专家组成员
辽宁省药学会生化药学委员会副主任委员
沈阳市药学会理事；学术委员会委员；生化药学委员会主任委员
“沈阳药科大学学报”编委
“药物生物技术”编委
三、研究方向
利用生物化学、分子生物学理论、技术，以药用动物等生物原材料为起始物，针对神经系统(癫痫、惊厥；镇痛)、肿瘤、心脑血管(抗血栓；促凝血)疾病的蛋白质类国内外创新药物理论基础、应用基础、开发及产业化；固定化酶工程的应用基础与产业化；基因工程应用基础与产业化；生物技术在药学、中药学的应用等研究领域和科研方向。生化药物、生物制品、基因工程药物等产业化及生产技术支持。
己申报多项药用生物分子及其获得方法的中国发明专利，其中有的申报了PCT（国际专利公约）专利以及美国和欧盟专利。在美国GenBank登录新基因多项。
1.蛋白质化学:
①国内外创新药用蛋白先导化合物分离纯化、制备工艺
②国内外创新药用蛋白先导化合物筛选及其结构解析
③国内外创新药物临床前研究
④蛋白质结构与功能关系
⑤蛋白、多肽、酶类药物生产工艺
2.生物技术:
①外源目的基因在原核细胞的可溶性高效表达
②外源目的基因在酵母细胞的高效表达
③药物靶向融合体的研究
④生物技术在药学、中药学研究中的应用
3.药物作用机理-信号传导:
①从细胞膜离子通道等角度，研究创新药物作用机理
②从蛋白质组学角度，研究创新药物作用机理
4.固定化酶工程:
①异构体(手性)药物及中间体转化特定酶的筛选、制备及其固定化
②药物及中间体转化特定酶的筛选、制备及其固定化
5.其它:
①生化药物、生物制品稳定性、分析和检测
②解决蛋白质、多肽、酶这类生化药物、基因工程药物生产工艺、产量、成本、 质量等生产技术
四、科研工作
先后主持承担“国家科技部十二五重大新药创制课题”、“国家自然科学基金”、“国家科技攻关”、“中国博士后科学基金”、“霍英东教育基金会―高等院校青年教师基金”、“省市重点科研、自然科学基金”等课题二十余项。在国内外期刊等发表八十余篇论文，其中三十余篇是SCI收录的杂志。出版各类教材、专著十本，如，人民卫生出版社出版的全国高等医药院校教材《生物化学》、《药学分子生物学》，中国医药科技出版社出版的《生物化学实验》，现代药学高级丛书《实用生物制药学》。
五、招生方向
①微生物与生化药学专业方向招收博士及硕士研究生
②生物学专业方向招收硕士研究生
③中药生物技术专业方向招收硕士研究生
六、主要代表性论文：
1、Li Deng, Hong-Xia Zhang, Yu Wang, Rong Zhang, Xue Wen, Yong-Bo Song, Yong-Shan Zhao, Lin Ma, Chun-Fu Wu, Jing-Hai Zhang＊. Site-Directed Mutagenesis of BmK AGP-SYPU1: The Role of Two Conserved Tyr (Tyr5 and Tyr42) in Analgesic Activity, Protein J, 2014, 33:157-164
2、Guang-Ying Du, Hai-Bo Zhu, Peng-Fei Yu, Hong-Bo Wang, Jie He, Liang Ye, Feng-Hua Fu, Jing-Hai Zhang＊, Jing-Wei Tian. SMND-309 promotes angiogenesis in human umbilical vein endothelial cells through activating erythropoietin receptor/STAT3/VEGF pathways, European Journal of Pharmacology, 2013, 700(1-3):173-180
3、Yong Shan Zhao, Yue Qiu Wang, Yang Xu, Yong Cui, Rong Zhang, Yan Feng Liu, Yong Bo Song, Hong Xing Zhang, Jing Hai Zhang＊. Molecular mechanism of Bmk9 -Nav1.8 channel recognition explored by docking and molecular dynamic simulations, Molecular Simulation
4、Qian Liu, Yao Jin, Kung Wang, Xiang-xue Meng, Yang Yang, Zhuo Yang, Yong-shan Zhao, Ming-yi Zhao, Jing-hai Zhang＊. Study of the binding residues between β1 and β3 subunits of sodium channel, CRB, 2014, 337(2):73-77
5、Jian-hua Shao, Yong Cui, Ming-yi Zhao, Yan-feng Liu＊, Chun-fu Wu, Jing-hai Zhang＊＊. Purification, characterization, and bioactivity of a new analgesic-antitumor peptide from Chinese scorpion Buthus martensii Karsch, Peptides, 2014
6、Yong-shan Zhao, Rong Zhang, Yang Xu, Yong Cui, Yan-feng Liu, Yong-bo Song, Hong-xing Zhang, Jing-hai Zhang＊. The role of glycine residues at the C-terminal peptide segment in antinociceptive activity: a molecular dynamics simulation, J Mol Model, 2013, 19: 1295-1299
7、Yu Wang, Yong-bo Song, Guang-zhao Yang, Yong Cui, Yong-shan Zhao, Yan-feng Liu, Yan Ma, Chun-fu Wu, Jing-hai Zhang. Arginine Residues in the C-terminal and their Relationship with the Analgesic Activity of the Toxin from the Chinese Scorpion Buthus martensii Karsch (BmK AGP-SYPU1), Appl Biochem Biotechnol, 2012 Sep;168(2):247-255
8、Rui Ma, Yong Cui, Ying Zhou, et al. Location of the analgesic domain in Scorpion toxin BmK AGAP by mutagenesis of disulfide bridges, BBRC, 2010, 394: 330-334
9、Yong Cui, Gui-Li Guo, Lin Ma, et al. Structure and function relationship of toxin from Chinese scorpion Buthus martensii Karsch (BmKAGAP) Gaining insight into related sites of analgesic activity, Peptides, 2010, 31(6): 995-1000
10、Yong Cui, Yanfeng Liu, Qiqing Chen, et al. Genomic cloning, characterization and statistical analysis of an antitumor-analgesic peptide from Chinese scorpion Buthus martensii Karsch, Toxicon, 2010, 56: 432-439
11、Yong Cui, Yong-Bo Song, Lin Ma, Yan-Feng Liu, Guo-Dong Li, Chun-Fu Wu, Jing-Hai Zhang. Site-directed Mutagenesis of the Toxin from the Chinese Scorpion Buthus martensii Karsch Insight into Sites Related to Analgesic Activity, Arch. Pharm. Res., 2010, 33(10): 1633-1639
12、Rong Zhang, Yong Cui, Xi Zhang, et al. Soluble expression, purification and the role of C-terminal glycine residues in scorpion toxin BmK AGP-SYPU2, BMB Reports, 2010, 43(12): 801-806
13、Yu Wang, Li Wang, Yong Cui, et al. Purification, characterization and functional expression of a new peptide with an analgesic effect from Chinese scorpion Buthus martensii Karsch (BmK AGP-SYPU1), BIOMEDICAL CHROMATOGRAPHY, 2011, 25: 801-807
14、Yong Cui, Gui-Li Guo, Yan-Feng Liu, et al. Construction of three different recombinant scorpion fusion proteins with bifunctional activitity, IJBB, 2011, 48(3): 141-147
15、Yueqiu Wang, Zhihui Hao, Jianhua Shao, et al. The role of Ser54 in the antinociceptive activity of BmK9, a neurotoxin from the scorpion Buthus martensii Karsch, Toxicon, 2011, 58(6-7): 527-532
16、Rong Zhang, Zhuo Yang, Yanfeng Liu, Yong Cui, Jinghai Zhang＊. PURIFICATION, CHARACTERIZATION AND cDNA CLONING OF AN ANALGESIC PEPTIDE FROM THE CHINESE SCORPION Buthus martensii Karsch (BmK AGP-SYPU2), Molecular Biology, 2011, 45(6): 879-885
17、Yong-Bo Song, Lin Ma, Wen-Yao Yang, Jian Wang, Mao-Sheng Cheng, Chun-Fu Wu and Jing-Hai Zhang＊. Study of the binding residues between ANEPⅡ and insect sodium channel receptor. Comptes Rendus Biologies 2010 Sep; 33 (9): 637-641
18、Jun Zhu, Jian Wang, Mao-Sheng Cheng, Jing-Hai Zhang＊. Dinucleotides docking to scorpion polypeptide toxins: A molecular modeling method for protein functional site recognition. Biochemical and Biophysical Research Communications 2009, 378: 157-161
19、W. Li, B. Du, T. Wang, S. Wang, J. Zhang＊. Kaempferol induces apoptosis in human HCT116 colon cancer cells via the Ataxia-Telangiectasia Mutated-p53 pathway with the involvement of p53 Upregulated Modulator of Apoptosis. Chem Biol Interact 177 (2009) 121-127
20、Yanfeng Liu, Zaiguo Zhang, Yingzi Mao, Yong Cui, Nan Hu, Ying Wang, Jinghai Zhang＊. Production and antitumor efficacy of recombinant Buthus martensii Karsch AGAP, ASIAN JOURNAL OF TRADITIONAL MEDICINES 2009, 4(6): 228-233
21、Jun Zhu, Jian Wang, Zhen-Cheng Su, Qin Li, Mao-Sheng Cheng, and Jing-Hai Zhang＊. Identification of ssDNA Aptamers Specific for Anti-neuroexcitation Peptide III and Molecular Modeling Studies: Insights into Structural Interactions. Archives of Pharmacal Research 2008, 31(9): 1120-1128
22、Jian-Hua Shao, Yue-Qiu Wang, Xiao-Yan Wu, Rui Jiang, Rong Zhang, Chun-Fu Wu, Jinghai Zhang＊. Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch. Biotechnology Letters 2008, 30: 23-29
|